Bacterial Morphology and Structure م.م رنا مشعل

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1 Bacterial Morphology and Structure م.م رنا مشعل

2 SIZE OF BACTERIA Unit for measurement : Micron or micrometer, μm: 1μm=10-3 mm Size: Varies with kinds of bacteria, and also related to their age and external environment.

3 Shape of Bacteria Cocci: sphere, 1μm Bacilli: rods, μm in width -3 μm in length Spiral : 1~3 μm in length and μm in width

4 Cocci Bacilli Spiral

5 Structure of Bacteria

6 Essential structures cell wall cell membrane Cytoplasm nuclear material Special structures Capsule Flagella Pili Spore

7 Cell wall Location: outermost portion nm in thickness, 10%-25% of dry weight.

8 1884: Christian Gram: First publication for the Gram stain method)

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10 Functions of Cell Wall Maintaining the cell's characteristic shapethe rigid wall compensates for the flexibility of the phospholipid membrane and keeps the cell from assuming a spherical shape Countering the effects of osmotic pressure Providing attachment sites for bacteriophages Providing a rigid platform for surface appendages- flagella, fimbriae, and pili all anchored to the wall and extend beyond it Play an essential role in cell division Be the sites of major antigenic determinants of the cell surface

11 Wall-less forms of Bacteria. When bacteria are treated with: 1) enzymes that are lytic for the cell wall e.g. lysozyme or 2) antibiotics that interfere with biosynthesis of peptidoglycan, wall-less bacteria are often produced. Usually these treatments generate non-viable organisms. Wall-less bacteria that can not replicate are referred to as protoplasts (if an outer membrane is not present).

12 Cell membrane

13 Function of Cell membrane a. Selective permeability and transport of solutes into cells b. Electron transport and oxidative phosphorylation c. Site of biosynthesis of DNA, cell wall polymers and membrane lipids.

14 Mesosomes Mesosomes are specialized structures formed by convoluted invaginations of cytoplasmic membrane.

15 Cytoplasm Composed largely of water, together with proteins, nucleic acid, lipids and small amount of sugars and salts Ribosomes: numerous, 15-20nm in diameter with 70S; Ribosomes are the protein synthesizing factories of the cell. They translate the information in mrna into protein sequences. distributed throughout the cytoplasm; sensitive to streptomycin and erythromycin site of protein synthesis

16 Plasmid Plasmids are: Small, Circular, Extrachromosomal, Double-stranded DNA molecules They are capable of self-replication and Contain genes that confer some properties, such as antibiotic resistance,virulence factors Plasmids are not essential for cellular survival.

17 Inclusions of Bacteria Inclusions are aggregates of various compounds that are normally involved in storing energy reserves or building blocks for the cell. Inclusions accumulate when a cell is grown in the presence of excess nutrients and they are often observed under laboratory conditions.

18 Nucleus Lacking nuclear membrane, absence of nucleoli, hence known as nucleic material or nucleoid, one to several per bacterium. Bacterial chromosome Single Circular Compacted

19 Capsules and slime layers These are structures surrounding the outside of the cell envelope. When more defined, they are referred to as a capsule when less defined as a slime layer. They usually consist of polysaccharide; They are not essential to cell viability and some strains within a species will produce a capsule, whilst others do not.

20 Capsules and slime layers

21 Function of Capsules and slime layers(1) Attachment :These structures are thought to help cells attach to their target environment. Streptococcus mutans produces a slime layer in the presence of sucrose. This results in dental plaque and many bacteria can stick to tooth surfaces and cause decay once S. mutans forms a slime layer. Vibrio cholerae, the cause of cholera, also produces a glycocalyx which helps it attach to the intestinal villi of the host.

22 Function of Capsules and slime layers(2) Protection from phagocytic engulfment. Bacterial pathogens are always in danger of being "eaten" by phagocytes. Streptococcus pneumoniae, when encapsulated is able to kill 90% of infected animals, when non-encapsulated no animals die. The capsule has been found to protect the bacteria by making it difficult for the phagocyte to engulf the microbe.

23 Function of Capsules and slime layers(3) Resistance to drying. Capsules and slime layers inhibit water from escaping into the environment.

24 Function of Capsules and slime layers(4) Reservoir for certain nutrients. Glycocalyx will bind certain ions and molecules. These can then be made available to the cell.

25 Flagella Flagella consist of a number of proteins including flagellin The diameter of a flagellum is thin, 20 nm, and long with some having a length 10 times the diameter of cell. Due to their small diameter, flagella cannot be seen in the light microscope unless a special stain is applied. Bacteria can have one or more flagella arranged in clumps or spread all over the cell. Mono/trichous Amphi/trichous Lopho/trichous Peri/trichous

26 Flagella Used in Identification of Bacteria Contribute to Pathogenesis Motility of bacteria

27 Pili Pili are hair-like projections of the cell, They are known to be receptors for certain bacterial viruses. Chemical nature is pilin Sex pili: longer and coarser, Conjugation, as it is called, is one explanation for the rapid spread of drug resistance in many different species of bacteria.

28 Spores The mechanisms that account for this include the dehydration of the protoplast and the production of special proteins that protect the spores DNA. Are capable of detecting their environment and under favorable nutrient conditions germinating and returning to the vegetative state.

29 Spore Used in Identification of Bacteria Contribute to Pathogenesis Resistance to adverse environmental conditions